Peptide Dosing Guide

Complete dosing protocols for all major anti-aging peptides, including injection techniques, storage, cycling, and safety guidelines.

Peptide Dosing Protocols

BPC-157 Dosing

• Standard dose: 250-500mcg daily
• For acute injuries: 500mcg twice daily
• Injection site: Subcutaneous near injury site or abdominal
• Cycle length: 4-12 weeks
• Half-life: Approximately 4 hours

Best for: Injury recovery, gut health, tissue repair, inflammation

TB-500 Dosing

• Standard dose: 2-2.5mg twice weekly
• Loading phase: 2.5mg twice weekly for 4-6 weeks
• Maintenance: 2.5mg once weekly
• Cycle length: 6-12 weeks

Best for: Faster injury healing, flexibility, tissue regeneration

NAD+ Dosing

• Oral supplements (NMN/NR): 250-500mg daily
• IV/Injection: 100-500mg daily or weekly
• Best time: Morning on empty stomach
• Cycle: Can be used continuously

Best for: Anti-aging, energy, cognitive function, cellular repair

GLP-1 Agonists (Semaglutide, Tirzepatide) Dosing

• Semaglutide: Weekly injection, starts at 0.25mg
• Tirzepatide: Weekly injection, starts at 2.5mg
• Requires prescription: Must consult healthcare provider

Best for: Weight loss, blood sugar control, metabolic health

Ipamorelin Dosing

• Standard dose: 100-200mcg 2-3 times daily
• Best time: Before bed, morning, or post-workout
• Cycle: 8-12 weeks, then 4 weeks off
• Often stacked with: CJC-1295 for synergistic effects

Best for: Muscle growth, recovery, anti-aging, sleep

CJC-1295 Dosing

• Standard dose: 100mcg 1-2 times daily
• With Ipamorelin: 100mcg of each, 2x daily
• Best time: Before bed

Best for: Growth hormone optimization, muscle growth, anti-aging

How to Inject Peptides: Complete Guide

Supplies You'll Need

• Peptide vials (stored in refrigerator)
• Bacteriostatic water for reconstitution
• Insulin syringes (29-31 gauge)
• Alcohol prep pads
• Sharps container for disposal

Reconstitution Steps

1. Remove peptide vial and bacteriostatic water from refrigerator
2. Wipe both vial tops with alcohol swab
3. Draw appropriate amount of bacteriostatic water (typically 2mL for 5mg vial)
4. Slowly inject water into peptide vial, aiming at the side wall
5. Gently swirl to dissolve – do not shake
6. Store reconstituted peptide in refrigerator

Injection Steps

1. Wash hands thoroughly
2. Wipe injection site with alcohol swab
3. Draw prescribed amount into syringe
4. Pinch skin and insert needle at 45-90 degree angle
5. Inject slowly and steadily
6. Withdraw needle and apply gentle pressure
7. Rotate injection sites to prevent irritation

Injection Sites

• Abdomen: Most common, 2 inches from navel
• Thigh: Front of thigh, halfway between knee and hip
• Upper arm: Back of arm (may need assistance)

Peptide Storage Guidelines

• Lyophilized (powder): Refrigerate or freeze for long-term
• Reconstituted: Refrigerate (36-46°F / 2-8°C)
• Shelf life: Typically 30-60 days reconstituted
• Avoid: Direct sunlight, heat, freezing after reconstitution

Peptide Safety Guidelines

Do's

• Research thoroughly before starting
• Purchase from reputable vendors with third-party testing
• Start with low doses and assess tolerance
• Use sterile technique for all injections
• Keep detailed logs of doses, timing, and effects
• Store peptides properly
• Cycle peptides appropriately

Don'ts

• Don't exceed recommended doses
• Don't use if pregnant or breastfeeding
• Don't mix different peptides in same syringe (unless instructed)
• Don't use cloudy or discolored solutions
• Don't share needles or vials
• Don't ignore unusual side effects

Common Side Effects

Most peptides are well-tolerated, but side effects can occur:

• Injection site reactions: Redness, itching, swelling
• Headaches: Common with NAD+ and growth hormone secretagogues
• Water retention: Can occur with some peptides
• Fatigue: Possible during initial use
• Flu-like symptoms: Some users report initial effects

Managing Side Effects

• Reduce dose if side effects occur
• Stay well hydrated
• Ensure proper injection technique
• Rotate injection sites
• Take breaks between cycles

Cycling Peptides

General Cycling Guidelines

• Most peptides: 8-12 weeks on, 4 weeks off
• BPC-157/TB-500: Can run longer for injury recovery
• NAD+: Can be used continuously
• Growth hormone secretagogues: 12 weeks on, 4-6 weeks off

Why Cycle?

• Prevents receptor desensitization
• Maintains natural hormone production
• Reduces risk of side effects
• Allows body to reset

Stacking Peptides

Popular Stacks

• Recovery Stack: BPC-157 + TB-500 for injury healing
• Anti-Aging Stack: NAD+ + Ipamorelin + CJC-1295
• Performance Stack: Ipamorelin + CJC-1295
• Comprehensive Stack: BPC-157 + TB-500 + Ipamorelin

Stacking Guidelines

• Start with one peptide before adding others
• Understand each peptide's effects individually
• Adjust doses when stacking (often lower than individual use)
• Monitor for additive side effects

Next Steps: See our Getting Started Guide for choosing the right peptides for your goals, or visit our Trusted Vendors page to find quality peptide suppliers.